2

Materials

2.1

Cell Culture

2.1.1

HepG2 Tissue

Model

HepG2 hepatocellular carcinoma cells (ECACC).

6-well Alvetex^® insert (REPROCELL).

6-well plate.

Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher

Scientific).

Fetal bovine serum (FBS) (Thermo Fisher Scientific).

Penicillin–streptomycin (Thermo Fisher Scientific).

L-Glutamine (Thermo Fisher Scientific).

Trypsin-EDTA (Thermo Fisher Scientific).

Phosphate Buffered Saline.

Sterile Forceps.

Stirred Bioreactor Apparatus.

2.1.2

Intestinal Tissue

Model

Stromal Compartment

Human neonatal dermal fibroblasts (HDFn, Thermo Fisher

Scientific).

Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher

Scientific).

Fetal bovine serum (FBS, Thermo Fisher Scientific).

L-glutamine (Thermo Fisher Scientific).

Nonessential amino acids (NEAA, Thermo Fisher Scientific).

Penicillin–streptomycin solution (Thermo Fisher Scientific).

Transforming growth factor β1 (TGFβ1, PeproTech).

Ascorbic acid (Sigma-Aldrich).

Trypsin-EDTA (Thermo Fisher Scientific).

Phosphate buffered saline.

Sterile forceps.

Epithelial Compartment

Human epithelial colorectal adenocarcinoma cells (Caco-2, The

European Collection for Authenticated Cell Cultures).

Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher

Scientific).

Fetal bovine serum (FBS, Thermo Fisher Scientific).

L-Glutamine (Thermo Fisher Scientific).

Nonessential amino acids (NEAA, Thermo Fisher Scientific).

Penicillin–streptomycin solution (Thermo Fisher Scientific).

Sterile forceps.

Stirred bioreactor apparatus.

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